Project 1.
1. Revealing the genomic information of four major anti-cancer medicinal plants
- de novo genome assembly
- Gene prediction and gene annotation
- Genetic variation and bio-marker development
2. Therapeutic mechanisms of the medicinal components of plants
- Cell type specific gene expression analysis using single cell RNA sequencing
- Cell culture methods to purify plant medicinal ingredients with efficiency and scale
- Metabolite analysis and molecular mechanism studies of herbal medicine ingredients
Project 2.
1. Discovery of novel CRISPR/Cas systems through metagenome analysis
- Candidate screening by microbiome analysis in cattle, sheep, and camels
- CRISPR/Cas sequence analysis based on relevant database construction
- Efficiency confirmation of novel Cas candidates in animal systems
- Optimization of novel Cas systems for plants
2. Evolutionary studies of bacterial CRISPR/Cas systems
- Characteristics of system subtypes with a CRISPR/Cas sequence database
- Pipeline development for CRISPR/Cas system analysis
- Discovery of novel CRISPR/Cas systems
- Evolutionary approach for a comprehensive CRISPR/Cas system
Project 3.
1. Tissue culture technologies for four major anti-cancer medicinal resources
- Establishment of plant tissue culture screening systems
- Optimization of plant tissue culture systems
- Plant cell identity verification experiments
- Cell culture technologies for medicinal ingredient production, optimized for plants
2. Application of novel CRISPR/Cas systems in plants
- Discovery and application of plant-suitable CRISPR/Cas systems
- Expanded utilization of medicinal plants
- Attempt to mass-produce useful medicinal substances with a bioreactor
